Polyubiquitin binding and cross-reactivity in the USP domain deubiquitinase USP21 Supplementary Material
نویسندگان
چکیده
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, UK Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 303222, USA Section of Structural Biology, The Institute of Cancer Research, 237 Fulham Road, London, UK $ Current address: Laboratory of Biochemistry and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA # Current address: Institute of Biochemistry, ETH Hönggerberg HPM G10, CH-8093 Zürich
منابع مشابه
Polyubiquitin binding and cross-reactivity in the USP domain deubiquitinase USP21.
Modification of proteins by ubiquitin (Ub) and Ub-like (Ubl) modifiers regulates a variety of cellular functions. The ability of Ub to form chains of eight structurally and functionally distinct types adds further complexity to the system. Ub-specific proteases (USPs) hydrolyse polyUb chains, and some have been suggested to be cross-reactive with Ubl modifiers, such as neural precursor cell exp...
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The mechanisms that regulate the nucleocytoplasmic localization of human deubiquitinases remain largely unknown. The nuclear export receptor CRM1 binds to specific amino acid motifs termed NESs (nuclear export sequences). By using in silico prediction and experimental validation of candidate sequences, we identified 32 active NESs and 78 inactive NES-like motifs in human deubiquitinases. These ...
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Ubiquitination is a reversible modification that influences a broad range of physiological processes. There are approximately 90 DUBs encoded in the human genome, of which 79 are predicted to have catalytic activity. We have tagged 66 DUBs with GFP and systematically surveyed their sub-cellular distribution, identifying enzymes specific to the nucleus, plasma membrane, secretory and endocytic p...
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Ubiquitination is a reversible modification that influences a broad range of physiological processes. There are approximately 90 deubiquitinases (DUBs) encoded in the human genome, of which 79 are predicted to have catalytic activity. We tagged 66 DUBs with green fluorescent protein and systematically surveyed their subcellular distribution, identifying enzymes specific to the nucleus, plasma m...
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Nanog is a master pluripotency factor of embryonic stem cells (ESCs). Stable expression of Nanog is essential to maintain the stemness of ESCs. However, Nanog is a short-lived protein and quickly degraded by the ubiquitin-dependent proteasome system. Here we report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog, and therefore maintains the protein level of Na...
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